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‣ Value of Prenatal Diagnosis and Early Postnatal Diagnosis of Congenital Toxoplasmosis: Retrospective Study of 110 Cases

Robert-Gangneux, Florence; Gavinet, Marie-Françoise; Ancelle, Thierry; Raymond, Josette; Tourte-Schaefer, Claudine; Dupouy-Camet, Jean
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Artigo de Revista Científica
Publicado em /09/1999 Português
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We reviewed the files of 110 women with Toxoplasma seroconversion during pregnancy. Prenatal diagnosis was attempted for 94 women by amniotic fluid sampling. Toxoplasma gondii was detected by PCR, with or without tissue culture and mouse inoculation. The early neonatal diagnostic procedure included placental testing by PCR and/or mouse inoculation, cord blood serological testing, and comparison of maternal and newborn antibodies by Western blotting (WB). Serological follow-up of the infants was conducted during the first year of life or until the diagnosis of congenital toxoplasmosis (CT) could be ruled out. Congenital infection was diagnosed in 27 individuals (20 live births) in the prenatal and/or neonatal period. The sensitivity and specificity of prenatal diagnosis were 81 and 100%, respectively. Placental examination was positive for 66.7% of individuals with CT and was always negative for neonates without CT. Cord blood serology detected immunoglobulin M (IgM) and/or IgA in 80% of infected newborns, with respective specificities of 91.2 and 87.7%. By WB we detected bands on IgG and IgM blots recognized by the newborn serum but not by the maternal serum (neosynthesized IgG and/or IgM) for 88.2% of infected infants within the first 2 months of life with a specificity of 100%. Early postnatal diagnosis was negative for 2 of the 20 neonates with CT. Both of these newborns had a negative prenatal diagnosis and were asymptomatic...

‣ Lack of Utility of Specific Immunoglobulin G Antibody Avidity for Serodiagnosis of Reactivated Toxoplasmosis in Immunocompromised Patients

Mechain, Bénédicte; Garin, Yves Jean-François; Robert-Gangneux, Florence; Dupouy-Camet, Jean; Derouin, Francis
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Artigo de Revista Científica
Publicado em /07/2000 Português
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The avidities of Toxoplasma-specific immunoglobulin G serum antibodies were measured in immunocompromised patients presenting with cerebral or extracerebral toxoplasmosis and/or serological reactivation. Since avidity remained high and stable in 39 of 40 patients with toxoplasmosis and 27 of 28 patients with serological reactivation, we conclude that this test cannot help diagnose toxoplasmosis in these patients.

‣ Detection of Aspergillus Galactomannan: Comparison of an Enzyme-Linked Immunoassay and a Europium-Linked Time-Resolved Fluoroimmunoassay

Paugam, A.; Sarfati, J.; Romieu, R.; Viguier, M.; Dupouy-Camet, J.; Latgé, J. P.
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Artigo de Revista Científica
Publicado em /10/1998 Português
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With a view to improving the sensitivity of serological detection of Aspergillus galactomannan (GM), a europium-linked time-resolved fluoroimmunoassay was developed. This method was compared to an enzyme-linked immunosorbent assay using a peroxidase-conjugated detector antibody. No increase in the sensitivity of the detection of GM standards was seen with the europium-based fluoroimmunoassay.

‣ Mycetoma of the Foot Caused by Fusarium solani: Identification of the Etiologic Agent by DNA Sequencing

Yera, H.; Bougnoux, M. E.; Jeanrot, C.; Baixench, M. T.; De Pinieux, G.; Dupouy-Camet, J.
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Artigo de Revista Científica
Publicado em /04/2003 Português
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We report a case of Fusarium solani mycetoma of the foot that could not be diagnosed by culture, but was correctly identified after amplification and sequence analysis of fungal internal transcribed spacers 1 and 2 and 5.8S ribosomal DNA regions.

‣ Investigation of immunofluorescence cross-reactions against Trichinella spiralis by western blot (immunoblot) analysis.

Robert, F; Weil, B; Kassis, N; Dupouy-Camet, J
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /09/1996 Português
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Immunofluorescence cross-reactions in Trichinella spiralis serodiagnosis are sometimes difficult to identify. We compared the results of an indirect immunofluorescence assay and the profiles obtained by Western blot (immunoblot) analysis for three groups of patients: 10 T. spiralis-infected patients, 10 patients with autoimmune diseases, and 7 patients with parasitic diseases other than trichinellosis. The degree of immunofluorescence cross-reaction was variable. Western blotting allowed us to differentiate Trichinella infection from other parasitic diseases. In 3 of 10 serum samples from patients with autoimmune diseases, bands which had the same sizes as Trichinella bands were observed, and they could correspond to shared epitopes such as heat shock proteins.

‣ Development and Evaluation of a Western Blot Kit for Diagnosis of Human Trichinellosis

Yera, Hélène; Andiva, Shakir; Perret, Catherine; Limonne, Denis; Boireau, Pascal; Dupouy-Camet, Jean
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Artigo de Revista Científica
Publicado em /09/2003 Português
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We evaluated industrially prepared Western blot strips designed to avoid the cross-reactions observed with indirect immunofluorescence and enzyme-linked immunosorbent assays used for the serodiagnosis of trichinellosis. The antigen preparations were crude extracts of Trichinella spiralis. The Western blot profile characteristic of trichinellosis was characterized by comparing 60 sera from patients infected by Trichinella to 11 sera from healthy subjects, 51 sera from patients with other proven parasitic diseases (cysticercosis, schistosomiasis, strongyloidosis, fascioliasis, toxocariasis, liver amebiasis, anisakiasis, filariasis, toxoplasmosis, hydatidosis, or malaria), and 23 sera from patients with autoantibodies. Specific 43- to 44-kDa and 64-kDa bands were obtained with all of the sera from 51 patients with acute trichinellosis, in 4 out of 9 patients at the early stages of the disease, and in only 1 control patient, who had suspected anisakiasis and in whom trichinellosis could not be ruled out by muscle biopsy.

‣ Use of random amplified polymorphic DNA as a typing method for Candida albicans in epidemiological surveillance of a burn unit.

Robert, F; Lebreton, F; Bougnoux, M E; Paugam, A; Wassermann, D; Schlotterer, M; Tourte-Schaefer, C; Dupouy-Camet, J
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /09/1995 Português
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Burn patients are particularly exposed to deep-seated nosocomial infections caused by Candida species. Superficial carriage of C. albicans is a potential source of infection and dissemination, and typing methods could be useful to trace the different isolates. We report the use of random amplified polymorphic DNA to type isolates of C. albicans in the Hôpital Cochin burn unit. This molecular typing method, which is based on PCR with arbitrary short primers, was evaluated on a panel of 32 C. albicans strains isolated from various anatomical sites of unrelated patients, and the strains showed 22 different patterns. Random amplified polymorphic DNA was then used in the epidemiological surveillance of the patients in the burn unit over a 9-month period. Seven patterns were identified among 84 isolates from 18 patients. One pattern (pattern A) corresponding to isolates from 7 of the 18 patients (68% of isolates) predominated throughout the 9-month study, while some strains with other profiles were isolated only once. Some profiles appeared to show a particular geographic pattern within the unit, suggesting transmission from room to room. These results underline the importance of fungal surveillance in such patients and the need to inform nursing staff of measures to prevent the spread of Candida spp. from patient to patient.

‣ Congenital toxoplasmosis due to maternal reinfection during pregnancy.

Gavinet, M F; Robert, F; Firtion, G; Delouvrier, E; Hennequin, C; Maurin, J R; Tourte-Schaefer, C; Dupouy-Camet, J
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /05/1997 Português
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A case of congenital toxoplasmic chorioretinitis was diagnosed (specific-immunoglobulin G [IgG] and -IgM comparative Western blot analysis) in a baby whose mother was immune during pregnancy. Maternal sera showed an increase in specific IgG and emergence of both IgM and IgA during pregnancy. The mother was probably reinfected through contact with kittens.

‣ Molecular epidemiology of nosocomial invasive aspergillosis.

Girardin, H; Sarfati, J; Traoré, F; Dupouy Camet, J; Derouin, F; Latgé, J P
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /03/1994 Português
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Moderately repeated DNA sequences were used to fingerprint strains of Aspergillus fumigatus isolated from patients with invasive aspergillosis and their hospital environment. Most strains sampled from the environment displayed different Southern blot hybridization patterns. A temporal survey of air contaminants showed that some strains can persist in the same environment for at least 6 months. Patients with invasive aspergillosis were infected by a single strain. In two patients, a nosocomial origin of infection was suggested.

‣ Detection of Toxoplasma gondii in venous blood from AIDS patients by polymerase chain reaction.

Dupouy-Camet, J; de Souza, S L; Maslo, C; Paugam, A; Saimot, A G; Benarous, R; Tourte-Schaefer, C; Derouin, F
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /07/1993 Português
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Detection of Toxoplasma gondii in blood by means of the polymerase chain reaction (PCR) may facilitate the diagnosis and follow-up of cerebral toxoplasmosis in patients with AIDS. We evaluated this approach with seven patients with tissue culture-proven parasitemia, 14 patients with presumptive cerebral toxoplasmosis, and 17 healthy human immunodeficiency virus-positive controls. Each sample of blood was assayed on three different occasions by a PCR assay based on detection of the gene encoding the P30 surface protein. A positive PCR diagnosis required positivity in at least two of the three PCR tests. None of the controls had a positive PCR diagnosis, but six of the seven patients with parasitemia did. Cerebral toxoplasmosis was confirmed in 13 of the 14 patients with a presumptive diagnosis; diagnosis by PCR was positive before treatment for 9 of these 13 patients, whereas tissue culture was positive for only 1 patient. During treatment, blood samples were taken from 14 patients at regular intervals until day 12. PCR diagnosis became negative on ethidium-stained gels, but persistent signals were observed after hybridization, in some cases, for up to 12 days after initiation of therapy. PCR on venous blood could thus be a sensitive and noninvasive method for the diagnosis of cerebral and disseminated toxoplasmosis in AIDS patients and could be a potential tool for monitoring the effects of treatment.

‣ Collaborative evaluation in seven laboratories of a standardized micromethod for yeast susceptibility testing.

Guinet, R; Nerson, D; de Closets, F; Dupouy-Camet, J; Kures, L; Marjollet, M; Poirot, J L; Ros, A; Texier-Maugein, J; Volle, P J
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /11/1988 Português
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The new micromethod for yeast susceptibility testing, MYCOTOTAL, was evaluated with 10 reference strains in seven laboratories. Ready-to-use microtitration plates and the same synthetic medium were used with two dilutions of imidazoles, flucytosine, and amphotericin B, permitting the categorization of each strain as susceptible, intermediate, or resistant. The results were compared with the MIC for each reference strain, and the repeatability and reproducibility were evaluated. The yeasts tested presenting different patterns of susceptibilities in reference MICs included six strains of Candida albicans, two strains of Candida tropicalis, one strain of Candida parapsilosis, and one strain of Torulopsis glabrata. For 4,200 antifungal agent-yeast results, the repeatability was 99.3% and the reproducibility was 96.3%. The correlation between the reference MICs and the category results was 91.5% for seven laboratories (and 92.7% for six laboratories excluding the laboratory which did not follow exactly the same protocol). We observed only 7.9% minor discrepancies, 0.5% (0.29% for six laboratories) major discrepancies, and 0.1% uninterpretable results. The percentages of concording results were similar for each strain and each antifungal agent tested. The overall results indicated that MYCOTOTAL was a reliable and reproducible method...

‣ Comparison of antibody, antigen, and metabolite assays for hospitalized patients with disseminated or peripheral candidiasis.

Bougnoux, M E; Hill, C; Moissenet, D; Feuilhade de Chauvin, M; Bonnay, M; Vicens-Sprauel, I; Pietri, F; McNeil, M; Kaufman, L; Dupouy-Camet, J
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /05/1990 Português
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Repeat serum samples from 22 patients with proven disseminated candidiasis and 42 with simple peripheral colonization were assayed for Candida antibodies by coelectrosyneresis, immunoprecipitation, and A and B immunofluorescence, for metabolites by D-arabinitol measurement, and for antigens by the mannan immunoassay and Cand-tec latex agglutination (mean number of samples tested, 2.5 per patient). For the antibody and metabolite assays, the results showed no statistical difference between the two groups. By contrast, the results of both antigen assays were positive for a significantly larger number of patients with disseminated candidiasis than of those with simple peripheral colonization. Results were regardless of whether the patients were neutropenic. They were not predictive of death. We calculated that the mannan antigen assay had 29% sensitivity and 97% specificity for the diagnosis of disseminated candidiasis. Likelihood ratios of a positive and a negative result of this test were 9.2 and 0.7, respectively, for this diagnosis. In the latex agglutination test, likelihood ratios were 2.5, 1.5, 1.6, and 0.3 when the test was positive for dilutions of 1/8, 1/4, and 1/2 and was negative, respectively.

‣ Immunopathogenesis of Toxoplasmosis in Pregnancy

Dupouy-Camet, Jean
Fonte: Hindawi Publishing Corporation Publicador: Hindawi Publishing Corporation
Tipo: Artigo de Revista Científica
Publicado em //1997 Português
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The immunopathogenesis of toxoplasmosis during pregnancy is not completely understood. This paper will try to discuss the most frequently asked questions about the immunopathogeny of congenital toxoplasmosis: differential virulence of Toxoplasma isolates, genetic susceptibility to infection, facilitation of placental transfer, models of congenital toxoplasmosis, and transmission in seropositive hosts. Most published data suggest a role of the genetic background of the host and of the parasite. Models of congenital toxoplasmosis have been evaluated, but it appears that the conclusion drawn would be barely appropriate to understand the pathogenesis in pregnant women.

‣ Contributions of Immunoblotting, Real-Time PCR, and the Goldmann-Witmer Coefficient to Diagnosis of Atypical Toxoplasmic Retinochoroiditis▿

Talabani, H.; Asseraf, M.; Yera, H.; Delair, E.; Ancelle, T.; Thulliez, P.; Brézin, A. P.; Dupouy-Camet, J.
Fonte: American Society for Microbiology (ASM) Publicador: American Society for Microbiology (ASM)
Tipo: Artigo de Revista Científica
Português
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Ocular toxoplasmosis is a major cause of posterior uveitis worldwide. The diagnosis is based mainly on ophthalmological examination. Biological diagnosis is necessary in atypical cases, and this requires aqueous humor sampling by anterior chamber paracentesis. We evaluated real-time PCR targeting the Toxoplasma gondii 529-bp repeat element, the Goldmann-Witmer coefficient (GWC), and immunoblotting for the diagnosis of toxoplasmic retinochoroiditis in 54 patients with atypical uveitis. The results of these biological tests, applied to paired aqueous humor-serum samples, were compared to the clinical findings. Combining either PCR or the GWC with immunoblotting increased the sensitivity to 73% or 70%, respectively. Together, PCR and the GWC had 80% sensitivity. If feasible, sensitivity can be increased by combining the three methods (85% sensitivity). The interval between symptom onset and anterior chamber paracentesis strongly influenced the detection of specific intraocular antibody synthesis. The sensitivity of the GWC increased from 45% to 56% when sampling was performed 10 days after symptom onset, and that of immunoblotting increased from 53% to 72% when puncture was performed 30 days after symptom onset. PCR analysis of aqueous humor samples detected toxoplasmic DNA in 55% of patients. In contrast to the results of immunoblotting and the GWC...

‣ Comics carnet : the graphic novelist as global nomad

Bader, Edward.
Fonte: Brock University Publicador: Brock University
Tipo: Electronic Thesis or Dissertation
Português
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An interdisciplinary approach is used to identify a new graphic novel genre, 'comics camet', and its key features. The study situates comics camet in a historical context and shows it to be the result of a cross-pollination between the American and French comics traditions. Comics camet incorporates features from other literary genres: journalism, autobiography, ethnography and travel writing. Its creators, primarily European rriales, document their experiences visiting countries that Europe has traditionally defined as belonging to the 'East'. A visual and narrative analysis, using theoretical perspectives derived from cultural and postcolonial studies, examines how comics camet represents the non-European other and identifies the genre's ideological assumptions. Four representative texts are examined: Joe Sacco's Palestine (2001), Craig Thompson's, Camet de Voyage (2004), Guy Delisle's Pyongyang (2005) and Mrujane Satrpi's Persespolis 2 (2004). The study concludes that the comics camet genre simultaneously reinforces and challenges stereotypical assumptions about non-European people and places.

‣ Estudo comparativo do uso da terra em unidades de produ??o familiar no Nordeste Paraense

MOREIRA, Aninha Melo
Fonte: Universidade Federal do Pará Publicador: Universidade Federal do Pará
Tipo: Dissertação de Mestrado
Português
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O contexto hist?rico de forma??o territorial do Nordeste Paraense, est? relacionado com os diversos per?odos de ocupa??o que a regi?o Amaz?nica sofreu, desde os prim?rdios da coloniza??o, passando pelos fluxos migrat?rios, a partir da d?cada de 1950, at? sua configura??o atual. O Nordeste Paraense ? composto pelas microrregi?es do Salgado, Bragantina, Camet?, Guam? e Tom?-A?u, ocupando cerca de 135 mil quil?metros quadrados, ou seja, 10,6% da superf?cie estadual, englobando 49 munic?pios, possuindo 1,8 milh?o de habitantes, 27% da popula??o estadual. Ap?s quase um s?culo de utiliza??o as ?reas de mata virgem fazem parte do passado. O padr?o de uso da terra baseia-se no manejo de parcelas de floresta secund?ria (capoeiras), em rota??o com culturas anuais e a implanta??o de culturas perenes e de pastagens. Neste contexto este trabalho objetivou compreender a din?mica do uso da terra em unidades de produ??o familiar, para assim subsidiar alternativas para o planejamento das propriedades. A pesquisa foi realizada em trinta e tr?s unidades, dispostas nos munic?pios de Bragan?a (apresenta uma ocupa??o mais antiga, com diferentes hist?ricos de ocupa??o e uso da terra, aproximadamente 300 anos), Capit?o Po?o (representa uma ocupa??o e uso intermedi?rios...

‣ Comparación entre técnicas de inoculación de Fusarium verticillioides en espigas de maíz

Poulsen Hornum,M; Ridao,A; Castaño,F
Fonte: RIA. Revista de investigaciones agropecuarias Publicador: RIA. Revista de investigaciones agropecuarias
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/12/2013 Português
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Se compararon Técnicas de Inoculación (TI) de Fusarium verticillioides en espigas de maíz a través del comportamiento de 15 cultivares frente a la Incidencia (I%) y Severidad (S%) de la podredumbre. Se realizó un ensayo en la localidad de Camet (Gral. Pueyrredón, Buenos Aires), en 2006/07, en el que las plantas recibieron las siguientes TI: 1) aspersión de una Suspensión de Inóculo (SI) sobre los Estigmas (ASE), 2) inyección de una SI en el Canal de los estigmas (ICE), 3) inserción de un palillo colonizado con micelio (IE) y 4) control, sin inoculación (C). Para la I%, se detectó interacción híbrido x TI. El método IE produjo mayor I% en todos los híbridos. En cambio, no hubo interacción híbrido x TI para S%, ni diferencia entre híbridos. Hubo correlación significativa según la I% según la infección natural ocurrida en Camet durante 2006/07 y la obtenida por ASE, ICE y C. Aunque ASE simuló mejor a una infección natural, los resultados de I% mostraron que ICE permitiría una mejor evaluación de la podredumbre.

‣ Playas de bolsillo en Mar chiquita provincia de Buenos Aires

Bértola,Germán R; Merlotto,Alejandra; Cortizo,Luis; Isla,Federico I
Fonte: Revista de la Asociación Geológica Argentina Publicador: Revista de la Asociación Geológica Argentina
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/06/2013 Português
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La costa norte de General Pueyrredon y sur de Mar Chiquita (Buenos Aires) está constituida por playas de bolsillo, desarrolladas entre cabos rocosos o entre espigones. Su dinámica mayormente erosiva está condicionada a fenómenos naturales episódicos (tormentas sudestadas) y a acciones antrópicas derivadas del drenaje de excesos hídricos o las alteraciones estacionales que hacen los concesionarios de balnearios. En los últimos años estas playas han sido objeto de desbalances originados por sucesivas obstrucciones de la deriva litoral originadas por campos de espigones y la reciente construcción de escolleras, requeridas para el tendido del emisario cloacal de Mar del Plata. Los efectos de la erosión exceden los sectores urbanizados al punto que se han estimado retrocesos de las escarpas de médano superiores a 1 m/año al norte de Mar Chiquita. Con el objeto de analizar los impactos (naturales y antrópicos) en relación a la dinámica de estas playas de bolsillo, se ejecutaron perfiles en 6 playas del litoral durante los intervalos 2004-2005 y 2009-2011. De la comparación de fotografías aéreas e imágenes modernas se pudo reconocer la magnitud de la erosión costera según los intervalos 1957-1987 y 1987- 2000. Los espigones han logrado disminuir efectos erosivos hacia el sur de Santa Clara...

‣ Bradysia aliciae sp. nov. (Diptera: Sciaridae) del Pleistoceno de Buenos Aires, Argentina

Ramírez,Leonardo C.; Alonso,Cecilia P.
Fonte: Revista de la Sociedad Entomológica Argentina Publicador: Revista de la Sociedad Entomológica Argentina
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/06/2014 Português
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Bradysia aliciae sp. nov. (Diptera: Sciaridae) es descripta a partir de un ala con un excepcional estado de preservación proveniente del afloramiento de Camet Norte en Buenos Aires, Argentina, con una antigüedad de 24.500 años A.P. El ejemplar es el primer díptero adulto identificable que proviene de dichos depósitos.

‣ Niveles de tefras en sedimentos continentales del Pleistoceno tardío-Holoceno (Provincia de Buenos Aires)

Gentile,Rodolfo O.; Ribot,Alejandro M.
Fonte: Intersecciones en antropología Publicador: Intersecciones en antropología
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/12/2001 Português
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Se caracterizan niveles de tefras en tres localidades de la Provincia de Buenos Aires: Olavarría, Tandil y Camet Norte. El nivel de Olavarría, fue identificado en el Miembro Guerrero (Pleistoceno tardío) de la Formación Luján, el de Tandil intercala en sedimentos de la Formación Las Animas (Pleistoceno tardío - Holoceno), mientras que el tercero se localiza en la Facies Camet Norte (Formación Santa Clara) cuya edad fuera asignada al Pleistoceno tardío. Considerando dataciones radiocarbónicas realizadas por otros autores, el nivel de tefras de Olavarría se ha depositado entre 26.930 + 860 años 14C A.P. y 9.990-10.280 años cal A.P, pudiendo haberse acumulado alrededor de 19.320 - 20.470 años cal A.P. y 20.650 - 21.760 años cal A.P. El nivel de tefras de la Facies Camet Norte y el del Miembro Guerrero, están alojados en unidades litoestratigráficas correlacionables, aunque no se ha establecido si ambos niveles representan un mismo evento temporal. Las tefras de Tandil se acumularon en algún momento del Pleistoceno tardío Holoceno (preconquista). El componente más abundante de las tefras es vidrio volcánico de composición ácida (riolítica hasta dacítica) y en menor proporción, más básica, observándose en los depósitos que las contienen una importante participación de materiales piroclásticos.