Página 1 dos resultados de 21998 itens digitais encontrados em 0.029 segundos

‣ Surface grafting of carboxylic groups onto thermoplastic polyurethanes to reduce cell adhesion

Alves, P.; Ferreira, P.; Kaiser, Jean-Pierre; Salk, Natalie; Bruinink, Arie; Sousa, Hermínio C. de; Gil, M. H.
Fonte: Elsevier Publicador: Elsevier
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
663.57016%
The interaction of polymers with other materials is an important issue, being their surface properties clearly crucial. For some important polymer applications, their surfaces have to be modified. Surface modification aims to tailor the surface characteristics of a material for a specific application without affecting its bulk properties. Materials can be surface modified by using biological, chemical or physical methods. The aim of this work was to improve the reactivity of the thermoplastic polyurethane (TPU) material (Elastollan®) surface and to make its surface cell repellent by grafting carboxylic groups onto its surface. Two TPU materials were studied: a polyether-based TPU and a polyester-based TPU. The grafting efficiency was evaluated by contact angle measurements and by analytical determination of the COOH groups. Scanning electron microscopy (SEM) of the membranes surface was performed as well as cell adhesion tests. It was proved that the surfaces of the TPUs membranes were successfully modified and that cell adhesion was remarkably reduced.

‣ Interações moleculares na adesão celular em suportes sólidos e o efeito de fotossensibilizadores porfirínicos; Molecular interactions in cell adhesion on solid substrates and the effect of porphyrinic photosensitizers

Santos, Patrícia Araújo dos
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 28/03/2013 Português
Relevância na Pesquisa
676.8846%
A adesão celular está ligada à formação e disseminação de metástases, a principal causa de óbito de pacientes diagnosticados com câncer. O objetivo deste trabalho foi investigar in vitro o efeito de fotossensibilizadores na adesão celular. Foram utilizadas porfirinas comerciais (PpIX, CPpI, TSPP, TMPyP e Zn(II)TMPyP) e um fotossensibilizador sintetizado através da ligação de poli-L-lisina à protoporfirina IX (PLLPpIX). A adesão celular foi estudada por RICM, técnica que permite quantificar a área de contato entre uma célula e um substrato por binarização das imagens digitais utilizando limiares apropriados. A técnica foi padronizada e revelou dois regimes de adesão celular: um limitado e outro não limitado pela quantidade de proteína de adesão adsorvida na superfície. Neste último foi observada lise celular. Todos os fotossensibilizadores estudados foram capazes de aumentar a adesão celular na ausência de irradiação comparados ao controle sem fotossensibilizador, o que não havia sido observado nos ensaios de resistência à tripsinização normalmente utilizados para estudar o efeito de fotossensibilizadores na adesão celular. Quanto maior a anfifilicidade do fotossensibilizador, maior foi o efeito na adesão...

‣ Cell adhesion in free-standing multilayer films made of chitosan and alginate

Caridade, S. G.; Monge, C.; Mano, J. F.; Picart, C.
Fonte: Wiley Publicador: Wiley
Tipo: Conferência ou Objeto de Conferência
Publicado em /10/2012 Português
Relevância na Pesquisa
661.11055%
The method for preparing multilayer ultrathin films by the consecu- tive deposition of oppositely charged polyelectrolytes has gained tre- mendous recognition due the user friendly preparation, capability of incorporating high loads of different types of biomolecules in the films, fine control over the materials’ structure, and robustness of the products under ambient and physiological conditions. However the preparation of such films needs the assembly on a substrate and, sometimes, cannot be detached from it, which has limited the appli- cation of such films in areas as tissue engineering and regenerative medicine (TERM).Thus, the production of free-standing films is of extreme importance once it allows the direct experimental determi- nation of many physical properties of fundamental significance such as ion permeation and mechanical properties that can be tuned for real-world applications. In this work, we investigated the elaboration of free-standing multilayer films made of chitosan (CHI) and alginate (ALG), by detaching a polyelectrolyte multilayer film from its under- lying substrate without any postprocessing step. The conditions for optimized film growth were investigated. The adhesion of C2C12 myoblast cells on the CHI/ALG membrane was assessed by cytoskele- tal and nuclear staining. A good cell adhesion and spreading was observed all over the surface. The results demonstrate the potential of such biocompatible free standing membranes made of CHI and ALG for applications in TERM.

‣ Schistosoma mansoni: host cell adhesion to the different stages of the parasite, in vivo

Melo,Alan L.; Pereira,Leógenes H.; Machado,Conceição R. S.
Fonte: Instituto de Medicina Tropical Publicador: Instituto de Medicina Tropical
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/06/1992 Português
Relevância na Pesquisa
662.5769%
The peritoneal cavity of laboratory mice was used to study the phenomenon of host cell adhesion to different evolutive stages of the Schistosoma mansoni (cercaria, adult worm, developing and mature eggs, miracidium, young and mature daughter sporocysts). Material recovered from the peritoneal cavity 30 and 180 min after the inoculation of each evolutive form was examined with the help of a stereomicroscope. The free swimming larvae (cercaria and miracidium), and the evolutive forms producing such larvae (mature egg and mature daughter sporocyst) elicited the host cell adhesion phenomenon. In all forms but cercariae the adherent cells remained as so till 180 minutes after inoculation

‣ RNA and DNA aptamers as potential tools to prevent cell adhesion in disease

Ulrich,H.; Alves,M.J.M.; Colli,W.
Fonte: Associação Brasileira de Divulgação Científica Publicador: Associação Brasileira de Divulgação Científica
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/03/2001 Português
Relevância na Pesquisa
667.6304%
Recent research has shown that receptor-ligand interactions between surfaces of communicating cells are necessary prerequisites for cell proliferation, cell differentiation and immune defense. Cell-adhesion events have also been proposed for pathological conditions such as cancer growth, metastasis, and host-cell invasion by parasites such as Trypanosoma cruzi. RNA and DNA aptamers (aptus = Latin, fit) that have been selected from combinatorial nucleic acid libraries are capable of binding to cell-adhesion receptors leading to a halt in cellular processes induced by outside signals as a consequence of blockage of receptor-ligand interactions. We outline here a novel approach using RNA aptamers that bind to T. cruzi receptors and interrupt host-cell invasion in analogy to existing procedures of blocking selectin adhesion and function in vitro and in vivo.

‣ Dissecting cell adhesion architecture using advanced imaging techniques

Morton, Penny E; Parsons, Maddy
Fonte: Landes Bioscience Publicador: Landes Bioscience
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
669.4451%
Cell adhesion to extracellular matrix proteins or to other cells is essential for the control of embryonic development, tissue integrity, immune function and wound healing. Adhesions are tightly spatially regulated structures containing over one hundred different proteins that coordinate both dynamics and signaling events at these sites. Extensive biochemical and morphological analysis of adhesion types over the past three decades has greatly improved understanding of individual protein contributions to adhesion signaling and, in some cases, dynamics. However, it is becoming increasingly clear that these diverse macromolecular complexes contain a variety of protein sub-networks, as well as distinct sub-domains that likely play important roles in regulating adhesion behavior. Until recently, resolving these structures, which are often less than a micron in size, was hampered by the limitations of conventional light microscopy. However, recent advances in optical techniques and imaging methods have revealed exciting insight into the intricate control of adhesion structure and assembly. Here we provide an overview of the recent data arising from such studies of cell:matrix and cell:cell contact and an overview of the imaging strategies that have been applied to study the intricacies and hierarchy of proteins within adhesions.

‣ Dual Antibody Functionalized Polyvinyl Alcohol and Alginate Hydrogels for Synergistic Endothelial Cell Adhesion

Rafat, Marjan
Fonte: Harvard University Publicador: Harvard University
Tipo: Thesis or Dissertation
Português
Relevância na Pesquisa
669.0548%
Motivated by the need to design minimally-invasive treatments for wide-necked cerebral aneurysms, we used computational modeling to assess aneurysm hemodynamics, examined in vitro cellular responses arising from mechanical and chemical stresses, and designed novel materials that cooperatively adhere to the endothelium. We first hypothesized that because aneurysm geometry plays an important role in hemodynamics, changes in flow patterns may affect the shear stress experienced on the aneurysm wall. We defined flow regimes based on aneurysm hemodynamic and geometric parameters, which may correlate with aneurysm rupture. Because of the direct contact between endothelial cells (ECs) and blood flow, we then evaluated how changes in hemodynamics and inflammatory cytokines affect the expression of cell adhesion molecules (CAMs) and matrix remodeling factors on ECs. We subsequently designed biomaterials that complement the dynamic EC surface and have the ability to conform to any geometry through in situ crosslinking. Antibody-conjugated hydrogels facilitated synergistic EC adhesion using cooperativity as an adhesion strategy. We optimized the presentation of antibodies to inflammatory CAMs on polyvinyl alcohol (PVA) and alginate hydrogels to achieve strong adhesion to inflamed ECs. We synthesized photocrosslinkable...

‣ Melanoma Cell Adhesion Molecule is Associated with Myogenicity in Multiple Progenitor Populations within Human Fetal Skeletal Muscle

Lapan, Ariya
Fonte: Harvard University Publicador: Harvard University
Tipo: Thesis or Dissertation
Português
Relevância na Pesquisa
661.8266%
Skeletal muscle (SkM) possesses an impressive ability to regenerate in response to injury or chronic disease. This regenerative capacity is attributed to its resident mononuclear myogenic progenitors. Previous studies have identified several types of myogenic progenitors within SkM, some of which are isolated by fluorescence activated cell sorting (FACS) using cell surface markers. Studies in our laboratory have identified melanoma cell adhesion molecule (MCAM) as a cell surface marker expressed by myogenic progenitors in human fetal SkM. However, the relationship between MCAM expression and the degree of myogenic commitment of distinct MCAM+ populations has not been elucidated. In the present study, subpopulations of MCAM+ cells were purified by FACS on the basis of Hoechst 33342 dye uptake. Specifically, MCAM+ side population (SP) was isolated by Hoechst exclusion and MCAM+ main population (MP) on Hoechst incorporation. Sorted populations were first optimized for growth in vitro since SkM SP cells are difficult to maintain in culture. In particular, Invitrogen’s StemPro® MSC SFM medium was found to support propagation of human fetal SkM SP cells with minimal differentiation. Following this optimization, sorted populations were assessed for expression of myogenic markers before and after propagation and then for fusion potential in vitro and engraftment potential in vivo. The MCAM+ subpopulations were found to express myogenic markers to a significantly greater extent than MCAM- subpopulations. Furthermore...

‣ Folliculin, the Product of the Birt-Hogg-Dube Tumor Suppressor Gene, Interacts with the Adherens Junction Protein p0071 to Regulate Cell-Cell Adhesion

Medvetz, Douglas A; Khabibullin, Damir; Hariharan, Venkatesh; Ongusaha, Pat P.; Goncharova, Elena A.; Schlechter, Tanja; Darling, Thomas N.; Hofmann, Ilse; Krymskaya, Vera P.; Liao, James K.; Huang, Hayden; Henske, Elizabeth Petri
Fonte: Public Library of Science Publicador: Public Library of Science
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
671.908%
Birt-Hogg-Dube (BHD) is a tumor suppressor gene syndrome associated with fibrofolliculomas, cystic lung disease, and chromophobe renal cell carcinoma. In seeking to elucidate the pathogenesis of BHD, we discovered a physical interaction between folliculin (FLCN), the protein product of the BHD gene, and p0071, an armadillo repeat containing protein that localizes to the cytoplasm and to adherens junctions. Adherens junctions are one of the three cell-cell junctions that are essential to the establishment and maintenance of the cellular architecture of all epithelial tissues. Surprisingly, we found that downregulation of FLCN leads to increased cell-cell adhesion in functional cell-based assays and disruption of cell polarity in a three-dimensional lumen-forming assay, both of which are phenocopied by downregulation of p0071. These data indicate that the FLCN-p0071 protein complex is a negative regulator of cell-cell adhesion. We also found that FLCN positively regulates RhoA activity and Rho-associated kinase activity, consistent with the only known function of p0071. Finally, to examine the role of Flcn loss on cell-cell adhesion in vivo, we utilized keratin-14 cre-recombinase (K14-cre) to inactivate Flcn in the mouse epidermis. The K14-Cre-Bhdflox/flox mice have striking delays in eyelid opening...

‣ Folliculin regulates cell–cell adhesion, AMPK, and mTORC1 in a cell‐type‐specific manner in lung‐derived cells

Khabibullin, Damir; Medvetz, Douglas A.; Pinilla, Miguel; Hariharan, Venkatesh; Li, Chenggang; Hergrueter, Anja; Laucho Contreras, Maria; Zhang, Erik; Parkhitko, Andrey; Yu, Jane J.; Owen, Caroline A.; Huang, Hayden; Baron, Rebecca M.; Henske, Elizabeth P
Fonte: Wiley Periodicals, Inc. Publicador: Wiley Periodicals, Inc.
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
671.3737%
Abstract Germline loss‐of‐function BHD mutations cause cystic lung disease and hereditary pneumothorax, yet little is known about the impact of BHD mutations in the lung. Folliculin (FLCN), the product of the Birt–Hogg–Dube (BHD) gene, has been linked to altered cell–cell adhesion and to the AMPK and mTORC1 signaling pathways. We found that downregulation of FLCN in human bronchial epithelial (HBE) cells decreased the phosphorylation of ACC, a marker of AMPK activation, while downregulation of FLCN in small airway epithelial (SAEC) cells increased the activity of phospho‐S6, a marker of mTORC1 activation, highlighting the cell type–dependent functions of FLCN. Cell–cell adhesion forces were significantly increased in FLCN‐deficient HBE cells, consistent with prior findings in FLCN‐deficient human kidney‐derived cells. To determine how these altered cell–cell adhesion forces impact the lung, we exposed mice with heterozygous inactivation of Bhd (similarly to humans with germline inactivation of one BHD allele) to mechanical ventilation at high tidal volumes. Bhd+/− mice exhibited a trend (P = 0.08) toward increased elastance after 6 h of ventilation at 24 cc/kg. Our results indicate that FLCN regulates the AMPK and mTORC1 pathways and cell–cell adhesion in a cell type–dependent manner. FLCN deficiency may impact the physiologic response to inflation‐induced mechanical stress...

‣ Optimising parameters for the differentiation of SH-SY5Y cells to study cell adhesion and cell migration

Dwane, Susan; Durack, Edel; Kiely, Patrick A
Fonte: BioMed Central Publicador: BioMed Central
Tipo: info:eu-repo/semantics/article; all_ul_research; ul_published_reviewed
Português
Relevância na Pesquisa
670.4912%
peer-reviewed; Background: Cell migration is a fundamental biological process and has an important role in the developing brain by regulating a highly specific pattern of connections between nerve cells. Cell migration is required for axonal guidance and neurite outgrowth and involves a series of highly co-ordinated and overlapping signalling pathways. The non-receptor tyrosine kinase, Focal Adhesion Kinase (FAK) has an essential role in development and is the most highly expressed kinase in the developing CNS. FAK activity is essential for neuronal cell adhesion and migration. Results: The objective of this study was to optimise a protocol for the differentiation of the neuroblastoma cell line, SH-SY5Y. We determined the optimal extracellular matrix proteins and growth factor combinations required for the optimal differentiation of SH-SY5Y cells into neuronal-like cells and determined those conditions that induce the expression of FAK. It was confirmed that the cells were morphologically and biochemically differentiated when compared to undifferentiated cells. This is in direct contrast to commonly used differentiation methods that induce morphological differentiation but not biochemical differentiation. Conclusions: We conclude that we have optimised a protocol for the differentiation of SH-SY5Y cells that results in a cell population that is both morphologically and biochemically distinct from undifferentiated SH-SY5Y cells and has a distinct adhesion and spreading pattern and display extensive neurite outgrowth. This protocol will provide a neuronal model system for studying FAK activity during cell adhesion and migration events.

‣ The fasciclin-like arabinogalactan proteins of arabidopsis. A multigene family of putative cell adhesion molecules

Johnson, K.; Jones, B.; Bacic, A.; Schultz, C.
Fonte: Amer Soc Plant Physiologists Publicador: Amer Soc Plant Physiologists
Tipo: Artigo de Revista Científica
Publicado em //2003 Português
Relevância na Pesquisa
665.6048%
Fasciclin-like arabinogalactan proteins (FLAs) are a subclass of arabinogalactan proteins (AGPs) that have, in addition to predicted AGP-like glycosylated regions, putative cell adhesion domains known as fasciclin domains. In other eukaryotes (e.g. fruitfly [Drosophila melanogaster] and humans [Homo sapiens]), fasciclin domain-containing proteins are involved in cell adhesion. There are at least 21 FLAs in the annotated Arabidopsis genome. Despite the deduced proteins having low overall similarity, sequence analysis of the fasciclin domains in Arabidopsis FLAs identified two highly conserved regions that define this motif, suggesting that the cell adhesion function is conserved. We show that FLAs precipitate with β-glucosyl Yariv reagent, indicating that they share structural characteristics with AGPs. Fourteen of the FLA family members are predicted to be C-terminally substituted with a glycosylphosphatidylinositol anchor, a cleavable form of membrane anchor for proteins, indicating different FLAs may have different developmental roles. Publicly available microarray and expressed sequence tag data were used to select FLAs for further expression analysis. RNA gel blots for a number of FLAs indicate that they are likely to be important during plant development and in response to abiotic stress. FLAs 1...

‣ The role of deubiquitylating enzymes in cell-cell adhesion and development.

Millard, Susan
Fonte: Universidade de Adelaide Publicador: Universidade de Adelaide
Tipo: Tese de Doutorado Formato: 155773 bytes; 1550097 bytes; 1918368 bytes; 831006 bytes; 111646 bytes; application/pdf; application/pdf; application/pdf; application/pdf; application/pdf
Publicado em //2005 Português
Relevância na Pesquisa
670.8591%
Ubiquitylation is a versatile post-translational modification that participates in regulation of protein stability, via proteasomal and lysosomal degradative pathways, regulation of membrane protein internalisation and other trafficking events, and regulating the biological activity of some proteins independent of degradation. The diverse functions of ubiquitylation as a post- translational protein modification allow speculation that regulation of protein ubiquitylation status may be of crucial importance during the dynamic process of development. A screen of known, and suspected, ubiquitin pathway enzymes was designed to test this hypothesis. Whole mount in situ hybridisation was conducted on early post implantation mouse embryos to determine expression patterns of the ubiquitin pathway enzyme targets. This screen was not pursued in depth due to difficulties in resolving doubts regarding the sensitivity of the method and the validity of weak ubiquitous staining patterns. The FAM deubiquitylating enzyme is a known developmentally regulated ubiquitin pathway enzyme, and although believed to antagonise the conjugation of ubiquitin to specific substrates its cell biology remains poorly characterised. In different cellular contexts FAM has been reported to localise to points of cell-cell contact or to endosomes...

‣ Immunhistologische Analyse von Zelladhäsions- und Matrixmolekülen bei Nierenzellkarzinomen; Immunohistological analysis of cell adhesion and matrix molecules on renal cell carcinoma

Widmann, Susanne
Fonte: Universidade de Tubinga Publicador: Universidade de Tubinga
Tipo: Dissertação
Português
Relevância na Pesquisa
666.6285%
In dieser Arbeit wurde untersucht, ob bei renalen Karzinomen ein pathologisch verändertes Expressionsmuster von Adhäsionsmolekülen in Abhängigkeit vom Tumorstadium, vom zellulären Malignitätsgrad und der Histologie aufgezeigt werden kann. E- und N-Cadherin, die in der normalen Niere im Bereich distaler Tubulusanteile und der Sammelrohre bzw. auf Zellen des proximalen Tubulusepithels selektiv zu finden sind, wurden im Tumorgewebe in den meisten Fällen gleichzeitig exprimiert. Drei Tumore waren für beide Moleküle negativ. Bei einigen entdifferenzierten Tumoren wechselte die Expression von einer umschriebenen Verteilung auf der Zelloberfläche zu einer diffusen Anfärbung auch des Zytoplasmas. Die Mehrzahl der NZK zeigte keine VE-Cadherin-Expression. VE-Cadherin konnte im normalen Nierengewebe auf Endothelzellen verschiedener Blutgefäße, interstitieller Kapillaren und Glomeruli, im Tumorgewebe nur im Bereich von fokal gruppierten Gefäßen detektiert werden. Die meisten Endothelien waren in den NZK vermutlich als Hinweis für ihre geringe Ausreifung für VE-Cadherin negativ. P-Cadherin mit einer beschränkten Expression auf wenigen proximalen Tubuluszellen, mesangialen Zellen in Glomeruli und auf der Intima größerer Gefäße in der Normalniere...

‣ Bedeutung des Zelladhäsionsmoleküls Neurofascin für Prozesse der Synapsenbildung und -Stabilisierung im in vitro und in vivo Modell; Role of cell adhesion molecule neurofascin in synapse formation and stabilization in vitro and in vivo

Trinks, Sabine
Fonte: Universidade de Tubinga Publicador: Universidade de Tubinga
Tipo: Dissertação
Português
Relevância na Pesquisa
662.5769%
Affektive Störungen, wie Depressionen, Angsterkrankungen oder das Posttraumatische Stresssyndrom nehmen in der heutige Zeit einen immer höheren Stellenwert ein. Molekulare Mechanismen, die diesen Erkrankungen zugrunde liegen, sind nur unzureichend bekannt. Stress wurde bereits mehrfach als mögliche Ursache molekularer Veränderungen und Netzwerkstörungen beschrieben. Ebenso wurde Zelladhäsionsmolekülen eine große Bedeutung bei affektiven Störungen zugeschrieben. Neurofascin (NF) gehört der Ig-Superfamilie der Zelladhäsionsmoleküle an. Seine Lokalisation ist hauptsächlich auf das Axoninitialsegment (AIS) neuronaler Zellen beschränkt. In dieser Arbeit wurde die Bedeutung der neuronalen Neurofascin-Isoformen (NF166/NF180/NF186) auf inhibitorische Synapsenbildung und -stabilisierung sowohl in vitro, als auch in vivo untersucht. Dabei konnten modulatorische Effekte auf das Gerüstprotein Gephyrin in primären neuronalen Zellen am AIS beobachtet werden. Außerdem zeigte die shRNA-vermittelte Reduktion der Neurofascinexpression im Gyrus dentatus juveniler Ratten eine Abnahme prä- und postsynaptischer Strukturen am AIS. Des Weiteren konnte juveniler Stress im ventralen Gyrus dentaus von Ratten einen Anstieg der NF180-Expression bewirken...

‣ Cell adhesion molecules in stromal corneal dystrophies

Nemeth, Gabor; Felszeghy, Szabolcs; Kenyeres, Annamaria; Szentmáry, Nora; Berta, Andras; Süveges, Ildiko; Módis, Laszlo
Fonte: Murcia : F. Hernández Publicador: Murcia : F. Hernández
Tipo: Artigo de Revista Científica Formato: application/pdf
Português
Relevância na Pesquisa
665.7256%
The aim of the present study was to investigate the expression pattern of different cell adhesion molecules in corneal stromal dystrophies. Fifteen corneal buttons from patients diagnosed with three different types of stromal corneal dystrophies and healthy corneas were investigated. Paraffin embedded sections were stained immunohistochemically with monoclonal antibodies against human intercellular adhesion molecule-1 (ICAM-1), endothelial selectin (Eselectin) and endothelial cadherin (E-cadherin) using the avidin-biotin-peroxidase-complex technique. The sections were compared to normal eye bank controls. In corneas from granular dystrophy patients ICAM-1 was expressed focally in epithelial cells and in keratocytes, and expressed diffusely in endothelial cells. In corneas from macular dystrophy patients diffuse epithelial staining was observed and the stromal and endothelial expression was found to be similar to that of granular dystrophy. In lattice dystrophy, only the epithelial cells and endothelium were intensively positive for ICAM-1. E-selectin was not present on any layer of the corneal specimens. E-cadherin was observed only in the epithelium of all three types of corneal dystrophies. Normal corneas did not express any of the investigated adhesion molecules. We found different expression patterns of adhesion molecules in corneas from stromal dystrophies. Our results suggest that adhesion molecules may be involved in the pathogenesis of corneal stromal dystrophies.

‣ Studies of endothelial and leukocyte cell adhesion molecules in renal transplantation / by Warwick L. Grooby.

Grooby, Warwick L.
Fonte: Universidade de Adelaide Publicador: Universidade de Adelaide
Tipo: Tese de Doutorado Formato: 271974 bytes; application/pdf
Publicado em //1996 Português
Relevância na Pesquisa
665.6048%
This thesis examines the expression of cell adhesion molecules on both endothelial cells (ECs) and circulating leukocytes, and investigates the role of these molecules in renal allograft rejection. The aims of the study are to establish an ovine model of renal allograft transplantation, to generate monoclonal antibodies (mAbs) against ovine endothelial cell adhesion molecules, to examine the efficacy of the mAbs in prolonging the survival of the sheep renal allografts and to study the kinetics of expression of cell adhesion molecules (CAMs) during cellular activation 'in vitro' and 'in vivo' during the onset of allograft rejection.; Thesis (Ph.D.)--University of Adelaide, Dept. of Medicine, 1996; Erratum is pasted on back end paper.; Bibliography: leaves 231-268.; xv, 268 leaves : ill. (chiefly col.) ; 30 cm.

‣ Evolutionarily Conserved Function of Huntingtin in Cellular Dynamics Related to Cell Adhesion and the Cytoskeleton

Thompson, Morgan Nicole
Fonte: Harvard University Publicador: Harvard University
Tipo: Thesis or Dissertation
Português
Relevância na Pesquisa
668.7585%
Huntington's disease (HD) is a rare, dominantly inherited neurodegenerative disorder characterized by progressive chorea, emotional and behavioral disturbances, and cognitive decline. The single, causative mutation is an expanded trinucleotide repeat of cytosine, adenosine, and guanine (CAG) of more than 37 residues in the HD gene (currently referred to as HTT). Genetic evidence suggests that the CAG repeat expansion results in a gain of huntingtin function. While huntingtin and its numerous interactors have been implicated in a variety of essential cellular processes, the role of the full-length, endogenous protein remains unclear. Multiple studies have implicated huntingtin in processes related to cytoskeletal structure and dynamics in HD patients and model organisms. However, alterations in cellular dynamics related to the cytoskeleton — including cell adhesion — have not been characterized in a comprehensive, rigorous manner. Using Mus musculus genetic models of the HD mutation and/or deficiency and a Dictyostelium discoideum genetic deficiency model, I have undertaken an investigation of evolutionarily conserved huntingtin function in the cytoskeleton and cell adhesion. The results of these studies support a role for huntingtin in cell-cell and cell-substrate adhesion...

‣ How to let go: pectin and plant cell adhesion

Daher, Firas Bou; Braybrook, Siobhan A.
Fonte: Frontiers Publicador: Frontiers
Tipo: Article; published version
Português
Relevância na Pesquisa
668.1451%
This is the final version of the article. It first appeared from Frontiers via http://dx.doi.org/10.3389/fpls.2015.00523; Plant cells do not, in general, migrate. They maintain a fixed position relative to their neighbours, intimately linked through growth and differentiation. The mediator of this connection, the pectin-rich middle lamella, is deposited during cell division and maintained throughout the cell?s life to protect tissue integrity. The maintenance of adhesion requires cell wall modification and is dependent on the actin cytoskeleton. There are developmental processes that require cell separation, such as organ abscission, dehiscence, and ripening. In these instances, the pectin-rich middle lamella must be actively altered to allow cell separation, a process which also requires cell wall modification. In this review, we will focus on the role of pectin and its modification in cell adhesion and separation. Recent insights gained in pectin gel mechanics will be discussed in relation to existing knowledge of pectin chemistry as it relates to cell adhesion. As a whole, we hope to begin defining the physical mechanisms behind a cells? ability to hang on, and how it lets go.; The writing of this review was carried out with the help of grant BB-L002884-1 (BBSRC...

‣ Schistosoma mansoni: adesão de células do hospedeiro aos diferentes estádios do parasito, in vivo; Schistosoma mansoni: host cell adhesion to the different stages of the parasite, in vivo

Melo, Alan L.; Pereira, Leógenes H.; Machado, Conceição R. S.
Fonte: Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo Publicador: Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; ; ; ; ; Formato: application/pdf
Publicado em 01/06/1992 Português
Relevância na Pesquisa
662.5769%
A cavidade peritoneal de camundongos foi utilizada para estudos de adesão celular a diferentes estádios evolutivos do Schistosoma mansoni (cercária, verme adulto, ovos imaturos e maduros, miracídio, esporocisto jovem e esporocisto maduro). O material recuperado da cavidade peritoneal 30 e 180 min após o inóculo, foi examinado com auxílio de estereomicroscópio. As formas livres (cercária e miracídio) e as formas evolutivas que produzem tais larvas (ovo maduro e esporocisto maduro) apresentam células do hospedeiro aderidas à superfície. Em todas as formas, exceto cercária, as células permanecem aderidas pelo menos até 180 min após o inóculo; The peritoneal cavity of laboratory mice was used to study the phenomenon of host cell adhesion to different evolutive stages of the Schistosoma mansoni (cercaria, adult worm, developing and mature eggs, miracidium, young and mature daughter sporocysts). Material recovered from the peritoneal cavity 30 and 180 min after the inoculation of each evolutive form was examined with the help of a stereomicroscope. The free swimming larvae (cercaria and miracidium), and the evolutive forms producing such larvae (mature egg and mature daughter sporocyst) elicited the host cell adhesion phenomenon. In all forms but cercariae the adherent cells remained as so till 180 minutes after inoculation