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‣ Triagem de enzimas associadas à biotransformação de hidrocarbonetos a partir de metagenoma de sedimentos contaminados com petroléo e metais pesados; Screening of Enzymes Related to Biotransformation of Hydrocarbons from Metagenome of Contaminated Sediments with Oil and Heavy Metals
‣ High Content Screening of a Kinase-Focused Library Reveals Compounds Broadly-Active against Dengue Viruses
‣ Triagem de alto desempenho para detecção de atividade de epoxido-hidrolases e monooxigenases utilizando celulas integras; High-throughput screening in enzyme assays for epoxide hydrolases and monooxygenases activity detection using whole cell
‣ Expression system of CotA-laccase for directed evolution and high-throughput screenings for the oxidation of high-redox potential dyes
‣ Automated high-throughput screening of carbon nanotube-based bio-nanocomposites for bone cement applications
‣ High-throughput screening for tendons and ligaments healing
‣ A high-throughput screening assay for distinguishing nitrile hydratases from nitrilases
‣ Novel technologies for high-throughput and high-content studies on zebrafish larvae
‣ Exploring the mesenchymal stem cell niche using high throughput screening
‣ A holistic high-throughput screening framework for biofuel feedstock assessment that characterises variations in soluble sugars and cell wall composition in Sorghum bicolor
‣ Infrared methods for high throughput screening of metabolites: food and medical applications
‣ Markierungsfreies Hochdurchsatzscreening mittels reflektometrischer Interferenzspektroskopie; Label Free High-Throughput Screening With Reflectometric Interference Spectroscopy
‣ Microfluidic Plastic Devices for Single-use Applications in High-Throughput Screening and DNA-Analysis
‣ Cell-based high-throughput screening assay for identification of G-protein-coupled receptors agonists and antagonists
‣ A high-throughput screening assay for distinguishing nitrile hydratases from nitrilases
‣ Incorporating High-Throughput Exposure Predictions With Dosimetry-Adjusted In Vitro Bioactivity to Inform Chemical Toxicity Testing
‣ Kinetic assay for high-throughput screening of in vitro transthyretin amyloid fibrillogenesis inhibitors
‣ Descoberta de novos fármacos anti-tumorais e anti-bacterianos de extratos vegetais brasileiros através de screening em larga escala; Discovery of new antitumoral and antibacterial drugs from brazilian plant extracts using high throughput screening
‣ Development and Application of a Mass Spectrometry-Based Assay for the High Throughput Analysis of Protein-Ligand Binding
Many of the biological roles of proteins are modulated through protein-ligand interactions, making proteins important targets for drug therapies and diagnostic imaging probes. The discovery of novel ligands for a protein of interest often relies on the use of high throughput screening (HTS) technologies designed to detect protein-ligand binding. The basis of one such technology is a recently reported mass spectrometry-based assay termed SUPREX (stability of unpurified proteins from rates of H/D exchange). SUPREX is a technique that uses H/D exchange and MALDI-mass spectrometry for the measurement of protein stabilities and protein-ligand binding affinities. The single-point SUPREX assay is an abbreviated form of SUPREX that is capable of detecting protein-ligand interactions in a high throughput manner by exploiting the change in protein stability that occurs upon ligand binding.
This work is focused on the development and application of high throughput SUPREX protocols for the detection of protein-ligand binding. The first step in this process was to explore the scope of SUPREX for the analysis of non-two-state proteins to determine whether this large subset of proteins would be amenable to SUPREX analyses. Studies conducted on two model proteins...